Staining of BALB/c splenocytes with 0.125 µg of Rat IgG2a κ Isotype Control FITC (cat. 11-4321) (open histogram) or 0.125 µg of Anti-Mouse CD98 FITC (filled histogram). Total viable cells were used for analysis.
Contents: Anti-Mouse CD98 FITC Catalog Number: 11-0981 Concentration: 0.5 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: RL388 Host/Isotype: Rat IgG2a, κ
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11-0981-81
50 ug
11-0981-82
100 ug
11-0981-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The RL388 monoclonal antibody reacts with mouse CD98, also known as 4F2 antigen and Ly-10. RL388 detects a disulfide-linked heterodimer complex (130 kDa) composed of a glycosylated heavy (86 kDa) and a nonglycosylated light (39 kDa) subunits. CD98 is expressed on the surface of activated lymphocytes and is involved in cell activation and calcium flux events.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The RL388 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The RL388 antibody has been tested by flow cytometric analysis of activated and resting mouse splenocyte cell suspensions. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
MacDonald HR, Lees RK, Bron C. 1985. Cell surface glycoproteins involved in the stimulation of interleukin 1-dependent interleukin 2 production by a subline of EL4 thymoma cells. I. Functional characterization by monoclonal antibodies. J Immunol. 135(6):3944-50. Luscher B, Rousseaux M, Lees R, MacDonald HR, Bron C. 1985.Cell surface glycoproteins involved in the stimulation of interleukin 1-dependent interleukin 2 production by a subline of EL4 thymoma cells. II. Structure, biosynthesis, and maturation. J. Immunol. 135(6):3951-7.
