Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-mouse/rat CD90.1 (Thy-1.1, Thy1.1)
Catalog Number: 11-0900
Sizes: 50 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HIS51
Isotype: Mouse IgG2a, κ

Surface staining of rat splenocytes with 0.03 μg FITC (left) anti-mouse/rat CD90.1 (HIS51) and PE (right) anti-mouse/rat CD90.1 (HIS51). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0900	Affinity Purified anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	N/A	N/A	FC IH/F
13-0900	Biotin anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	N/A	N/A	FC
11-0900	FITC anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	488	518	FC
12-0900	PE anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	488	575	FC
15-0900	PE-Cy5 anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	488	670	FC
17-0900	APC anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	633	660	FC
25-0900	Phycoerythrin-Cy7 (PE-Cy7) anti-rat/mouse CD90.1 (Thy-1.1, Thy1.1)	488	760	FC
45-0900	PerCP-Cy5.5 anti-mouse/rat CD90.1 (Thy-1.1, Thy1.1) (PE-Cy5.5 replacement)	488	690	FC
93-0900	eFluor^TM 605^NC anti-mouse/rat CD90.1 (Thy-1.1, Thy1.1)	350-500	605	FC
47-0900	Allophycocyanin-eFluor^TM 780 anti-mouse/rat CD90.1 (Thy-1.1, Thy1.1) (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-0900	eFluor^TM 450 anti-mouse/rat CD90.1 (Thy-1.1, Thy1.1) (Pacific Blue® replacement)	405	450	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HIS51 monoclonal antibody reacts with rat CD90 and cross-reacts with mouse CD90.1, a GPI-linked membrane molecule. In the rat, CD90 is expressed by hematopoietic stem cells, immature B cells, thymocytes, recent thymic emigrants, neurons, inflammed endothelia and other cell types. In the CD90.1-expressing mouse strains, PL and AKR, CD90 is expressed by early hematopoietic cells in the bone marrow, thymocytes and mature T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HIS51 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The HIS51 antibody has been tested by flow cytometric analysis of rat thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kroese, F. G., N. K. de Boer, et al. 1995. Identification and kinetics of two recently bone marrow-derived B cell populations in peripheral lymphoid tissues. Cell Immunol 162(2): 185-93.
Hosseinzadeh, H. and I. Goldschneider 1993. Recent thymic emigrants in the rat express a unique antigenic phenotype and undergo post-thymic maturation in peripheral lymphoid tissues. J Immunol 150(5): 1670-9.
Ishizu, A., H. Ishikura, et al. 1995. Thy-1 induced on rat endothelium regulates vascular permeability at sites of inflammation. Int Immunol 7(12): 1939-47.
Hermans, M. H. and D. Opstelten 1991. In situ visualization of hemopoietic cell subsets and stromal elements in rat and mouse bone marrow by immunostaining of frozen sections. J Histochem Cytochem 39(12): 1627-34.

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Cat. 11-4724 Fluorescein isothiocyanate (FITC) Mouse IgG2a, K Isotype Control