Contents: Fluorescein isothiocyanate (FITC) anti-rat CD86 (B7-2)
Catalog Number: 11-0860
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 24F
Isotype: Mouse IgG1, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Surface staining of LPS stimulated rat splenocytes with 0.25 μg FITC (left) anti-rat CD86 (24F), and PE (right) anti-rat CD86 (24F). Autofluorescence is indicated by open histogram. Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-0860 |
Affinity Purified anti-rat CD86 (B7-2, B72) |
N/A |
N/A |
FA
FC
IHC
IP
|
| 16-0860 |
Functional Grade* Purified anti-rat CD86 (B7-2, B72) |
N/A |
N/A |
FC
|
| 13-0860 |
Biotin anti-rat CD86 (B7-2, B72) |
N/A |
N/A |
FC
|
| 11-0860 |
FITC anti-rat CD86 (B7-2, B72) |
488 |
518 |
FC
|
| 12-0860 |
PE anti-rat CD86 (B7-2, B72) |
488 |
575 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 24F monoclonal antibody reacts with rat CD86, an ~90 kDa surface receptor, also known as B7-2. CD86 and CD80 are members of the B7 family of costimulatory molecules. CD86 is expressed by germinal center B cells and at low levels on macrophages, dendritic cells and non-lymphoid cells in the thymus. Its expression is upregulated on B cells through a variety of surface stimuli including LPS. In addition to CD80 (B7-1), CD86 is a counter-receptor for the T cell surface molecules CD28 and CD152 (CTLA-4). The interaction of CD86 with its ligands plays a critical role in T-B crosstalk, T cell costimulation, autoantibody production and Th2-mediated Ig production. The kinetics of upregulation of CD86 upon stimulation supports its major contribution during the primary phase of an immune response.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 24F antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 24F antibody has been tested by flow cytometric analysis of resting and activated rat splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Maeda, K., T. Sato, et al. 1997. Characterization of rat CD80 and CD86 by molecular cloning and mAb. Int Immunol 9(7): 993-1000.
Damoiseaux, J. G., H. Yagita, et al. 1998. Costimulatory molecules CD80 and CD86 in the rat; tissue distribution and expression by antigen-presenting cells. J Leukoc Biol 64(6): 803-9.
Trinite, B., C. Voisine, et al. 2000. A subset of cytolytic dendritic cells in rat. J. Immunol. 165(8): 4202-8
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