Contents: Fluorescein isothiocyanate (FITC) anti-human CD82
Catalog Number: 11-0829
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 53H5
Isotype: Mouse IgG2a, κ
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Staining of normal human peripheral blood cells with FITC Mouse IgG2a, K Iso Cntrl (cat. 11-4724) (open histogram) or FITC anti-human CD82 (53H5) (colored histogram). Cells in the lymphocyte gate were used for analysis.
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Description
The 53H5 monoclonal antibody reacts with human CD82, a 50-53 kDa protein belonging to the tetra-span (TM4) transmembrane proteins. CD82 is expressed broadly by the majority of hematopoietic lineages, but not erythrocytes. Activation of T and B lymphocytes upregulates CD82 expression. CD82 is also expressed by epithelial and endothelial cells and fibroblasts.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 53H5 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 53H5 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood mononuclear cells. This can be used at 20 μl (0.125 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).
References
Shibagaki N, Hanada K, Yamaguchi S, Yamashita H, Shimada S, Hamada H. Functional analysis of CD82 in the early phase of T cell activation: roles in cell adhesion and signal transduction. Eur J Immunol. 1998 Apr;28(4):1125-33. (53H5, FA,
PubMed)
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Cat.
11-4724 Fluorescein isothiocyanate (FITC) Mouse IgG2a, K Isotype Control
Human Cellular Antigens Chart