Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD71 (Transferrin Receptor)
Catalog Number: 11-0717
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: T56/14
Isotype: Mouse IgG1

Staining of U937 cells with FITC Mouse IgG1, K Iso Cntrl (cat. 11-4714) (open histogram) or FITC anti-human CD71 (T56/14) (colored histogram). Total viable cells were used for analysis.

Description

The T56/14 monoclonal antibody reacts with human CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The T56/14 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The T56/14 antibody has been pre-titrated and tested by flow cytometric analysis of 3-day PHA activated human PBMC. This can be used at 5 μl (1 μg) per million cells in a 100 μl total staining volume.