Contents: Fluorescein isothiocyanate (FITC) anti-human CD69
Catalog Number: 11-0699
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: FN50
Isotype: Mouse IgG1, κ
HLDA No.: IV A091
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Surface staining of PHA stimulated human PBMC with anti-human CD69 (FN50) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
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Description
The FN50 monoclonal antibody reacts with human CD69, also known as very early activation antigen (VEA). CD69 is approximately 30 kDa and is expressed on the cell-surface as a disulfide-linked dimer. CD69 is rapidly upregulated upon activation and expressed on lymphocytes, monocytes and platelets.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The FN50 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The FN50 antibody has been pre-titrated and tested by flow cytometric analysis of resting and 6-hour TPA-activated human PBMC. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume.
References
Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
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Cat.
11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control
Human Cellular Antigens Chart