Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD62P (P-selectin)
Catalog Number: 11-0628
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: AK-4
Isotype: Mouse IgG1, κ
HLDA No.: VI P-44

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0628	FITC anti-human CD62P (P-selectin)	488	518	FC
12-0628	PE anti-human CD62P (P-selectin)	488	575	FC
14-0628	Affinity Purified anti-human CD62P (P-selectin)	N/A	N/A	FA FC IH/F
16-0628	Functional Grade* Purified anti-human CD62P (P-selectin)	N/A	N/A	FA
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The AK-4 monoclonal antibody reacts with human CD62P, a 140 kDa transmembrane glycoprotein also known as P-Selectin. Upon activation, CD62P is rapidly transported from its cytoplasmic stores in the Weibel-Palade bodies of endothelial cells or the alpha-granules of platelets to the surface. CD62P initiates the adhesive interaction between endothelial cells and neutrophils and monocytes during the inflammatory reaction and is also involved in the interaction of platelets with monocytes and neutrophils.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The AK-4 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This AK-4 antibody has been pre-titrated and tested by flow cytometric analysis of resting and thrombin-activated human platelets. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Skinner MP, Lucas CM, Burns GF, Chesterman CN, Berndt MC. GMP-140 binding to neutrophils is inhibited by sulfated glycans. J Biol Chem. 1991 Mar 25;266(9):5371-4.
Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London.

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Cat. 11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control