Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD57
Catalog Number: 11-0577
Sizes: 25 tests, 100 tests
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: TB01 (TBO1)
Isotype: Mouse IgM
HLDA No.: V

Staining of normal human peripheral blood cells with APC anti-human CD3 (cat. 17-0038) and FITC Mouse IgM Iso Cntrl (cat. 11-4752) (open histogram) or FITC anti-human CD57 (TB01) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0577	Fluorescein isothiocyanate (FITC) anti-human CD57	488	518	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

This TB01 monoclonal antibody reacts with human CD57 (also known as HNK-1 and Leu-7), a 110-kDa cell surface glycoprotein expressed on a subset of natural killer (NK) cells and NK T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The TB01 (TBO1) antibody has been reported for use in flow cytometric analysis.

Applications Tested

The TB01 (TBO1) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 μl (1.0 μg) per 100μl blood (or per 1 million cells in 100 μl total staining volume).

References

Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Lanier LL, Le AM, Phillips JH, Warner NL, Babcock GF. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol. 1983 Oct;131(4):1789-96.

Related Products

Cat. 17-0038 APC anti-human CD3 (clone UCHT1)
Cat. 11-4752 FITC Mouse IgM Isotype Control