Contents: Fluorescein isothiocyanate (FITC) anti-human CD55
Catalog Number: 11-0559
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 143-30
Isotype: Mouse IgG1, κ
HLDA No.: IV NL-7
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Surface staining of normal human peripheral blood cells with with FITC mouse IgG1 isotype control (cat. 11-4714) (open histogram) or FITC anti-human CD55 (143-30)(colored histogram). Total viable cells were used for analysis.
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Description
The 143-30 monoclonal antibody reacts with human CD55, the 64-73 kDa molecule also known as DAF (Decay-Accelerating Factor). CD55 is a GPI-anchored single chain glycoprotein expressed on all cells in contact with serum including all hematopoietic cells and vascular endothelium; a soluble form of CD55 is also present in plasma and body fluids. CD55 is thought to play a role in the protection of cells from the damage by autologous complement via prevention of the amplification steps of the complement cascade. Antibodies against CD55 are mitogenic in the presence of phorbol esters.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 143-30 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 143-30 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (1 μg) per million cells in a 100 μl total staining volume.
References
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Related Products
Cat.
11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control
Human Cellular Antigens Chart