Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD51/61
Catalog Number: 11-0519
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 23C6
Isotype: Mouse IgG1, κ
HLDA No.: IV P18

Staining of M21 cells with FITC Mouse IgG1, K Iso Cntrl (cat. 11-4714) (open histogram) or FITC anti-human CD51/CD61 (23C6) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0519	Affinity Purified anti-human CD51/61	N/A	N/A	FC IH/F
16-0519	Functional Grade* Purified anti-human CD51/61	N/A	N/A	FA FC IH/F
13-0519	Biotin anti-human CD51/61	N/A	N/A	FC
11-0519	FITC anti-human CD51/61	488	518	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 23C6 monoclonal antibody reacts with the human CD51/CD61 dimer, also known as the integrin αv/β3. CD51, an ~120 kDa surface molecule can also non-covalently associate with other β subunits of the integrin family including β₁ (CD29), β₅ and β₆ to form receptors for extracellular matrix components. Heterodimers of CD51/CD61 are expressed by melanoma cells, endothelial cells and osteoclasts and at very low levels by platelets. The CD51/CD61 complex mediates adhesion to fibrinogen, fibronectin, vitronectin and thrombospondin.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 23C6 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 23C6 antibody has been pre-titrated and tested by flow cytometric analysis of human melanoma cell line and peripheral blood leukocytes. This can be used at 20 μl (1 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control