Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD50 (ICAM-3)
Catalog Number: 11-0507
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: TP1/25.1
Isotype: Mouse IgG2a

Staining of normal human peripheral blood cells with 0.5 μg of FITC Mouse IgG2a, K Iso Cntrl (cat. 11-4724) (open histogram) or 0.5 μg of FITC anti-human CD50 (TP1/25.1) (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0507	Affinity Purified anti-human CD50 (ICAM-3)	N/A	N/A	FC
11-0507	Fluorescein isothiocyanate (FITC) anti-human CD50 (ICAM-3)	488	518	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The TP1/25.1 monoclonal antibody reacts with human CD50, also known as intercellular adhesion molecule-3 (ICAM-3). ICAM-3 is an approximately 110-120 kDa type I transmembrane protein which interacts with CD11a/CD18 (LFA-1) and mediates adhesion and leukocyte chemotaxis. CD50 is expressed by hematopoietic cells, endothelial cells and epidermal Langerhans cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The TP1/25.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The TP1/25.1 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 μl per 100μl blood (or per 1 million cells in 100 μl total staining volume).

Related Products

Cat. 11-4724 Fluorescein isothiocyanate (FITC) Mouse IgG2a, K Isotype Control