Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD49b (Integrin alpha 2)
Catalog Number: 11-0498
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioY418 (Y418)
Isotype: Mouse IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0498	Affinity Purified anti-human CD49b (Integrin alpha 2)	N/A	N/A	FC
16-0498	Functional Grade* Purified anti-human CD49b (Integrin alpha 2)	N/A	N/A	FA FC
11-0498	Fluorescein isothiocyanate (FITC) anti-human CD49b (Integrin alpha 2)	488	518	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioY418 monoclonal antibody reacts with human CD49b, the 150 kDa integrin α2 subunit. The complex of CD49b non-covalently associated with integrin β1 (CD29), also known as VLA-2, is a receptor for collagen and laminin. This complex is expressed by lymphocytes, activated T cells, monocytes and platelets.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioY418 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioY418 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood. This can be used at 20 μl (.125 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

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Cat. 11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control