Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD47
Catalog Number: 11-0478
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 2D3
Isotype: Mouse IgG1, κ

Staining of normal human peripheral blood cells with 1 μg of FITC Mouse IgG1, K Iso Cntrl (cat. 11-4714) (open histogram) or FITC anti-human CD47 (2D3) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0478	Affinity Purified anti-human CD47	N/A	N/A	FC WB
11-0478	Fluorescein isothiocyanate (FITC) anti-human CD47	488	518	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody 2D3 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin. CD47 is a glycosylated five transmembrane protein with a small alternatively spliced cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with β3 integrins CD51/CD61 and CD41/CD61. Furthermore this interaction can mediate bi-directional signaling to modify neural synaptic activity and regulate the phagocytic activities of macrophages. CD47 is the receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin). Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex). Expression is also found in non-hematopoietic cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 2D3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 2D3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (1 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Subramanian S, Parthasarathy R, Sen S, Boder ET, Discher DE. Species- and cell type-specific interactions between CD47 and human SIRPalpha. Blood. 2006 Mar 15;107(6):2548-56. (2D3, FC)

Related Products

Cat. 11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control