Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-mouse CD47
Catalog Number: 11-0471
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: miap301
Isotype: Rat IgG2a, κ

Staining of BALB/c splenocytes with 0.5 μg of FITC Rat IgG2a Iso Cntrl (cat. 11-4321) (open histogram) or 0.5 μg of FITC anti-mouse CD47 (miap301) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0471	Affinity Purified anti-mouse CD47	N/A	N/A	FC IH/F IP WB
11-0471	Fluorescein isothiocyanate (FITC) anti-mouse CD47	488	518	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody miap301 reacts to CD47 also known as integrin-associated protein (IAP), and neurophilin. CD47 is a glycosylated five transmembrane protein with a small cytoplasmic domain. CD47 is involved in adhesion through interactions with SIRP (signal regulator protein) and is non-covalently associated with ß3 integrins CD51/CD61, CD41/CD61. It is also known to be the receptor for thrombospondin. T cell expression of CD47 can mediate activation or apoptosis (in the presence of high levels of thrombospondin). Expression is found in the majority of hematopoietic cells including T and B cells, monocytes, platelets and erythrocytes (as part of the Rh complex). Expression is also found in non-hematopoietic cells.

No crossreactivity is seen against human CD47.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This miap301 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This miap301 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Chang HP, Ma YL, Wan FJ, Tsai LY, Lindberg FP, Lee EH. Functional blocking of integrin-associated protein impairs memory retention and decreases glutamate release from the hippocampus. Neuroscience. 2001;102(2):289-96

Jiang P, Lagenaur CF, Narayanan V. Integrin-associated protein is a ligand for the P84 neural adhesion molecule. J Biol Chem. 1999 Jan 8;274(2):559-62.

Mouro-Chanteloup I, Delaunay J, et al. 2003. Evidence that the red cell skeleton protein 4.2 interacts with the Rh membrane complex member CD47. Blood 101(1):338-44. (miap301, WB, PubMed)

Chang HP, Lindberg FP, et al. 1999. Impaired memory retention and decreased long-term potentiation in integrin-associated protein-deficient mice. (miap301, WB, PubMed)

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Cat. 11-4321 FITC Rat IgG2a Isotype Control