Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD45RO
Catalog Number: 11-0457
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: UCHL1
Isotype: Mouse IgG2a, κ
HLDA No.: IV N31

Surface staining of normal human peripheral blood cells with anti-human CD45RO (UCHL1) PE (left), and PE-Cy5 (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0457	FITC anti-human CD45RO	488	518	FC
12-0457	PE anti-human CD45RO	488	575	FC
15-0457	PE-Cy5 anti-human CD45RO	488	670	FC
17-0457	Allophycocyanin (APC) anti-human CD45RO	633	660	FC
14-0457	Affinity Purified anti-human CD45RO	N/A	N/A	FC IHC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The UCHL1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The UCHL1 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

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Cat. 11-4724 Fluorescein isothiocyanate (FITC) Mouse IgG2a, K Isotype Control