Contents: Fluorescein isothiocyanate (FITC) anti-mouse CD45RC
Catalog Number: 11-0456
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: GL24
Isotype: Rat IgM, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
|
|
 
Surface staining of mouse splenocytes with 1 μg FITC (left) anti-mouse CD45RC (GL24) and PE (right) anti-mouse CD45RC (GL24). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
|
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The GL24 monoclonal antibody reacts with the exon C-dependent isoform of mouse CD45. This CD45 isoform is expressed by peripheral B cells and a population of CD4
+ T cells at high levels, on a majority of CD8
+ T cells at intermediate levels and by a subpopulation of mouse thymocytes. CD45 is a protein tyrosine phosphatase and serves an important role in leukocyte signal transduction.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The GL24 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The GL24 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Hathcock, K. S., G. Laszlo, et al. (1992).Expression of variable exon A-, B-, and C-specific CD45 determinants on peripheral and thymic T cell populations.
J Immunol 148(1): 19-28.
Related Products
Cat.
11-4341 FITC Rat IgM Isotype Control
Mouse Cellular Antigens Chart