Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)
Catalog Number: 11-0453
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: A20
Isotype: Mouse IgG2a, κ

Staining of BALB/c (left) and SJL (right) splenocytes with 0.25 μg FITC Mouse IgG Iso Cntrl or 0.25 μg FITC anti-mouse CD45.1 (A20) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0453	FITC anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	488	518	FC
12-0453	PE anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	488	575	FC
13-0453	Biotin anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	N/A	N/A	FC
14-0453	Affinity Purified anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	N/A	N/A	FC IP
15-0453	PE-Cy5 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	488	670	FC
17-0453	APC anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	633	660	FC
19-0453	Cy5 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	633	670	FC
25-0453	PE-Cy7 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	488	760	FC
27-0453	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	633	779	FC
35-0453	PE-Cy5.5 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	488	690	FC
45-0453	PerCP-Cy5.5 anti-mouse CD45.1 (SJL, old Ly5.2, new Ly5.1)	488	690	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains. The strains that express CD45.1 include SJL/J, DA, STS/A and RIII. CD45.1 is expressed by all leukocytes in these strains.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The A20 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The A20 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Shen, F-W. (1981). "Monoclonal antibodies to mouse lymphocyte differentiation alloantigens. In Monoclonal antibodies and T cell Hybridomas; Perspective and technical advances, eds. Hammerling, G.J., U. Hammerling, and J.F. Kearney": 25-31.

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