Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-rat CD45.2
Catalog Number: 11-0450
Sizes: 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HIS41
Isotype: Mouse IgG1

Staining of rat splenocytes with 0.06 µg FITC mouse IgG1 isotype control (cat.11-4719) (open histogram) or 0.06 µg FITC anti-rat CD45.2 (HIS41) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0450	Affinity Purified anti-rat CD45.2	N/A	N/A	FC IH/F
11-0450	FITC anti-rat CD45.2	488	518	FC
12-0450	PE anti-rat CD45.2	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HIS41 monoclonal antibody reacts with all isoforms of the rat CD45 molecule, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in signaling and activation of lymphocytes. HIS41 reacts with strains expressing the RT7.2 alloantigen (BUF, LOU, and WF), but not strains expressing the RT7.1 alloantigen (ACI, AO, BN, DA, DZB, LEW, MAXX, PVG, SHR, and WAG).

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HIS41 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The HIS41 antibody has been tested by flow cytometric analysis of LOU rat splenocyte suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kampinga, J., F.G.M. Kroese, G.H. Pol, D. Opstelten, H.G. Seijen, J.H.A. Boot, B. Roser, P. Nieuwenhuis, and R. Aspinall. 1990. RT7-defined alloantigens in rats are part of the leukocyte common antigen family. Scand. J. Immunol. 31:699-710.
Giezeman-Smits, K. M., A. Gorter, R. L. P. van Vlierberghe, J. D. H. v. Eendenburg, A. M. M. Eggermont, G. J. Fleuren, P. J. K. Kuppen. 1999. The Regulatory Role of CD45 on Rat NK Cells in Target Cell Lysis. J. Immunol. 163:71-76.
Butcher, G.W. 1987. A list of monoclonal antibodies specific for alloantigens of the rat. J. Immunogenet. 14:163-176.

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Cat. 11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control