Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD43 (Leukosialin, Sialophorin)
Catalog Number: 11-0439
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio84-3C1 (84-3C1)
Isotype: Mouse IgG1

Staining of with APC anti-human CD3 (UCHT1) (cat. 17-0038) and 0.1 μg of FITC Mouse IgG1, K Iso Cntrl (cat. 11-4714) (left) or FITC anti-human CD43 (84-3C1) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0439	Fluorescein isothiocyanate (FITC) anti-human CD43 (Leukosialin, Sialophorin)	488	518	FC
12-0439	Phycoerythrin (PE) anti-human CD43 (Leukosialin, Sialophorin)	488	575	FC
17-0439	Allophycocyanin (APC) anti-human CD43 (Leukosialin, Sialophorin)	633	660	FC
14-0439	Affinity Purified anti-human CD43 (Leukosialin, Sialophorin)	N/A	N/A	FC WB

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal antibody ebio84-3C1 reacts with CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which like many mucins can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes except resting B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio84-3C1 (84-3C1) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio84-3C1 (84-3C1) antibody has been pre-titrated and tested by flow cytometric analysis of peripheral blood mononuclear cells. This can be used at 20 μl (0.25 μg) per million cells in a 100 μl total staining volume.

References

Borche L, Lozano F, Vilella R, Vives J. CD43 monoclonal antibodies recognize the large sialoglycoprotein of human leukocytes. Eur J Immunol. 1987 Oct;17(10):1523-6.

Schlossman, S., L. Bloumsell, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-0431 Fluorescein isothiocyanate (FITC) anti-mouse CD43 (Leukosialin) (clone eBioR2/60)
Cat. 11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control