Contents: Fluorescein isothiocyanate (FITC) anti-human CD43 (Leukosialin, Sialophorin)
Catalog Number: 11-0439
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio84-3C1 (84-3C1)
Isotype: Mouse IgG1
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Staining of with APC anti-human CD3 (UCHT1) (cat. 17-0038) and 0.1 μg of FITC Mouse IgG1, K Iso Cntrl (cat. 11-4714) (left) or FITC anti-human CD43 (84-3C1) (right). Cells in the lymphocyte gate were used for analysis.
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Description
The monoclonal antibody ebio84-3C1 reacts with CD43, which is also known as leukosialin, galactoglycoprotein and sialophorin. CD43 is a sialomucin which like many mucins can have both adhesive and anti-adhesive functions. Expression of CD43 is found on most leukocytes except resting B lymphocytes. Proteolytic processing upon activation decreases surface expression. CD43 is involved in activation of T cells, B cells, NK cells, and monocytes. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio84-3C1 (84-3C1) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBio84-3C1 (84-3C1) antibody has been pre-titrated and tested by flow cytometric analysis of peripheral blood mononuclear cells. This can be used at 20 μl (0.25 μg) per million cells in a 100 μl total staining volume.
References
Borche L, Lozano F, Vilella R, Vives J. CD43 monoclonal antibodies recognize the large sialoglycoprotein of human leukocytes. Eur J Immunol. 1987 Oct;17(10):1523-6.
Schlossman, S., L. Bloumsell, et al. eds. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Related Products
Cat.
11-0431 Fluorescein isothiocyanate (FITC) anti-mouse CD43 (Leukosialin) (clone eBioR2/60)
Cat.
11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control
Human Cellular Antigens Chart