Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-mouse CD40
Catalog Number: 11-0402
Sizes: 50 ug, 100 ug, 500 ug, 1 mg
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HM40-3
Isotype: Armenian Hamster IgM, κ

Staining of BALB/c splenocytes with 0.5 μg FITC Armenian Hamster IgG Iso Cntrl (cat. 11-4444) (open histogram) or 0.5 μg FITC anti-mouse CD40 (HM40-3) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0402	Affinity Purified anti-mouse CD40	N/A	N/A	FA FC IHC
16-0402	Functional Grade* Purified anti-mouse CD40	N/A	N/A	FA FC
13-0402	Biotin anti-mouse CD40	N/A	N/A	FC
11-0402	FITC anti-mouse CD40	488	518	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HM40-3 monoclonal antibody reacts with mouse CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation. HM40-3 has been reported in the literature to cross-react with the rat CD40 antigen.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HM40-3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The HM40-3 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions and can be used at less than or equal to 1 μg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kashiwada, M., Y. Yagita, et al. 1996. Activation of mitogen-activated protein kinases via CD40 is distinct from that stimulated by surface IgM on B cells. Eur. J. Immunol. 26: 1451-58
Inaba, K., M. Witmer-Pack, et al. 1994. The tissue distrbution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro. J. Exp. Med. 180:1849-60.
Kawano, T., J. Cui, et al. 1997. CD1d-restricted and TCR-mediated activation of Va14 NKT cells by glycosylceramides. Science 278: 1626-9
Ferlin, W. G., E. Severinson, et al. 1996. CD40 signaling induces interleukin-4-independent IgE switching in vivo. Eur J Immunol 26(12): 2911-5.
Trinite, B., C. Voisine, et al. 2000. A subset of cytolytic dendritic cells in rat. J. Immunol. 165(8): 4202-8.

Related Products

Cat. 14-0401  Affinity Purified anti-mouse CD40 (clone 1C10)
Cat. 15-0401  PE-Cy5 anti-mouse CD40 (clone 1C10)
Cat. 16-0401  Functional Grade Purified anti-mouse CD40 (clone 1C10)
Cat. 17-0401  APC anti-mouse CD40 (clone 1C10)