Staining of BALB/c splenocytes with 0.5 µg of Armenian Hamster IgG Isotype Control FITC (cat. 11-4888) (open histogram) or 0.5 µg of Anti-Mouse/Rat CD40 FITC (filled histogram). Total viable cells were used for analysis.
Contents: Anti-Mouse/Rat CD40 FITC Catalog Number: 11-0402 Concentration: 0.5 mg/ml Formulation: Phosphate buffer pH 7.2, 500 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: HM40-3 Host/Isotype: Armenian Hamster IgM, κ
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Cat. No.
Size
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11-0402-81
50 ug
11-0402-82
100 ug
11-0402-85
500 ug
11-0402-86
1 mg
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The HM40-3 monoclonal antibody reacts with mouse and rat CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The HM40-3 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The HM40-3 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions and can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Kashiwada, M., Y. Yagita, et al. 1996. Activation of mitogen-activated protein kinases via CD40 is distinct from that stimulated by surface IgM on B cells. Eur. J. Immunol. 26: 1451-58 Inaba, K., M. Witmer-Pack, et al. 1994. The tissue distrbution of the B7-2 costimulator in mice: abundant expression on dendritic cells in situ and during maturation in vitro. J. Exp. Med. 180:1849-60. Kawano, T., J. Cui, et al. 1997. CD1d-restricted and TCR-mediated activation of Va14 NKT cells by glycosylceramides. Science 278: 1626-9 Ferlin, W. G., E. Severinson, et al. 1996. CD40 signaling induces interleukin-4-independent IgE switching in vivo. Eur J Immunol 26(12): 2911-5. Trinite, B., C. Voisine, et al. 2000. A subset of cytolytic dendritic cells in rat. J. Immunol. 165(8): 4202-8.
