Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD36
Catalog Number: 11-0369
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioNL07 (NL07)
Isotype: Mouse IgM

Staining of normal human peripheral blood cells with 0.25 μg of FITC Mouse IgM Iso Cntrl (cat. 11-4752) (open histogram) or FITC anti-human CD36 (NL07) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0369	Fluorescein isothiocyanate (FITC) anti-human CD36	488	518	FC
14-0369	Purified anti-human CD36	N/A	N/A	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal antibody eBioNL07 recognizes human CD36, which is a member of the class B scavenger receptor family. CD36 was originally identified as a platelet-membrane glycoprotein also called glycoprotein IV and a receptor for thrombospondin-1 (TSP-1) and extracellular matrix proteins. Binding to TSP-1 is in the CLESH (CD36 LIMP-II Emp sequence homology) domain of CD36. CD36 expression is broad and includes microvascular (but not large vessel) endothelium, adipocytes, skeletal muscle, dendritic cells, epithelia of the retina, breast, and intestine, smooth muscle cells, and hematopoietic cells, including erythroid precursors, platelets, monocytes/macrophages, DCs and megakaryocytes. Expression on platelets is absent on Nak^a negative donors. Unlike other scavenger receptor, CD36 binds LDL that has been exposed to “minimally” oxidizing conditions. CD36 is also a fatty acid translocase (FAT) necessary for the transport of long-chain fatty acids (LCFAs) and therefore may play a role in atherosclerosis.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioNL07 (NL07) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioNL07 (NL07) antibody has been pre-titrated and tested by flow cytometric analysis. This can be used at 20 μl (0.25 μg) per million cells in a 100 μl total staining volume.

References

Alessio M, Greco NJ, Primo L, Ghigo D, Bosia A, Tandon NN, Ockenhouse CF, Jamieson GA, Malavasi F. Platelet activation and inhibition of malarial cytoadherence by the anti-CD36 IgM monoclonal antibody NL07. Blood. 1993 Dec 15;82(12):3637-47. (NL07, FC, PubMed)

Alessio M, Ghigo D, Garbarino G, Geuna M, Malavasi F. Analysis of the human CD36 leucocyte differentiation antigen by means of the monoclonal antibody NL07. Cell Immunol. 1991 Oct 15;137(2):487-500. (NL07, FC, PubMed)

Gruarin P, Ulliers D, Thorne RF, Alessio M. Methionine 156 in the immunodominant domain of CD36 contributes to define the epitope recognized by the NL07 MoAb. Mol Cell Biochem. 2000 Nov;214(1-2):89-95. (NL07, PubMed)

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Cat. 11-4752 FITC Mouse IgM Isotype Control