Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD33
Catalog Number: 11-0339
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HIM3-4
Isotype: Mouse IgG1, κ
HLDA No.: V MA112

Surface staining of normal human peripheral blood cells with anti-human CD33 (HIM3-4) PE. Appropriate isotype controls were used (open histogram). Cells in the monocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0339	Affinity Purified anti-human CD33	N/A	N/A	FC
11-0339	FITC anti-human CD33	488	518	FC
12-0339	PE anti-human CD33	488	575	FC
15-0339	PE-Cy5 anti-human CD33	488	670	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The HIM3-4 monoclonal antibody reacts with human CD33, a 67 kDa member of the sialoadhesion family. CD33 is expressed by myelomonocytic precursors, monocytes, mast cells, and granulocytes. Hematopoietic stem cells and lymphocytes do not express this antigen. CD33 plays a role in sialic-acid dependent cell adhesion.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HIM3-4 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The HIM3-4 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (1 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-0338  FITC anti-human CD33 (clone WM-53 (WM53))
Cat. 12-0338  PE anti-human CD33 (clone WM-53 (WM53))
Cat. 14-0338  Affinity Purified anti-human CD33 (clone WM-53 (WM53))
Cat. 15-0338  Phycoerythrin-Cy5 (PE-Cy5) anti-human CD33 (clone WM-53 (WM53))
Cat. 11-4714  Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control