Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD33
Catalog Number: 11-0338
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: WM-53 (WM53)
Isotype: Mouse IgG1, κ
HLDA No.: VI M505

Staining of normal human peripheral blood cells with 0.5 µg of FITC mouse IgG1 isotype control (cat.11-4714) (open histogram) or FITC WM-53 (colored histogram). Cells in the monocyte (left) and granulocyte (right) gates were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0338	Affinity Purified anti-human CD33	N/A	N/A	FC IHC IP MIC
11-0338	FITC anti-human CD33	488	518	FC
12-0338	PE anti-human CD33	488	575	FC
15-0338	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD33	488	670	FC
25-0338	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD33	488	760	FC
56-0338	Alexa Fluor® 700 anti-human CD33 (APC-Cy5.5 Equivalent)	633	723	FC
45-0338	PerCP-Cy5.5 anti-human CD33 (PE-Cy5.5 replacement)	488	690	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The WM-53 monoclonal antibody reacts with human CD33, also known as GP67 and P67, a 67 kDa type I transmembrane glycoprotein that is a member of the Siglec (sialic acid-binding Ig superfamily lectin) family. It is highly specific to the hematopoietic compartment and is expressed on monocytes, activated T cells, granulocytes, myeloid progenitors, and mast cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The WM-53 (WM53) antibody has been reported for use in flow cytometric analysis.

Applications Tested

The WM-53 (WM53) antibody has been pre-titrated and tested by flow cytometric analysis of lysed whole blood. This can be used at 20 µl (0.5 μg) per million cells.

References

Favaloro EJ, Bradstock KF, Kabral A, Grimsley P, Berndt MC. 1987 Characterization of monoclonal antibodies to the human myeloid-differentiation antigen, 'gp67' (CD-33). Dis Markers. 5(4):215-25.

Related Products

Cat. 11-0339  FITC anti-human CD33 (clone HIM3-4)
Cat. 12-0339  PE anti-human CD33 (clone HIM3-4)
Cat. 14-0339  Affinity Purified anti-human CD33 (clone HIM3-4)
Cat. 15-0339  PE-Cy5 anti-human CD33 (clone HIM3-4)
Cat. 11-4714  Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control