Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-mouse siglec H - marker for IPC/pDC
Catalog Number: 11-0333
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio440c
Isotype: Rat IgG2b

Staining of SJL splenocytes. Left: PE anti-mo/hu B220 (RA3-6B2) (cat. 12-0452) and FITC 0.125 μg of FITC anti-mouse siglec H (eBio440c). Right: Costaining of AlexaFluor647 anti-mouse PDCA (cat. 51-3172) and FITC anti-mouse siglec H (eBio440c). Cells in the large forward scatter population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0333	Fluorescein isothiocyanate (FITC) anti-mouse siglec H - marker for IPC/pDC plasmacytoid DC	488	518	FC
51-0333	Alexa Fluor® 647 anti-mouse siglec H - marker for IPC/pDC plasmacytoid DC	633	668	FC
14-0333	Affinity Purified anti-mouse siglec H - marker for IPC/pDC plasmacytoid DC	N/A	N/A	FC IHC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal antibody eBio440c recognizes Siglec-H, a protein exclusively found on pDC (plasmacytoid dendritic cells) or type I IFN-producing cells (IPC) in the naïve mouse. Mouse IPC are typically PDCA+, CD11c+, CD11b-, B220+, and Ly-6C+, and are quick to respond to viruses. Siglec-H is a transmembrane protein of the Ig superfamily that like CD33 have been shown to bind sialic acid but lacks the characteristic cytoplasmic ITIM domain (immunoreceptor tyrosine based inhibitory motif). To overcome the lack of a cytoplasmic domain, Siglec-H associates with DAP12 thereby allowing for signal transduction. The eBio440c antibody has been shown to inhibit pDC function (inhbits IFNalpha secretion in response to CpG).

It has been observed that some mouse strains (such as SJL) have higher percentages of pDCs compared to C57BL/6.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio440c antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio440c antibody has been tested by flow cytometric analysis of SJL mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Amanda Blasius, William Vermi, Anne Krug, Fabio Facchetti, Marina Cella, and Marco Colonna. A cell-surface molecule selectively expressed on murine natural interferonα producing cells that blocks secretion of interferonα. Blood. 2004;103:4201-4206 (440c, FC, IH/F, FA PubMed)

Blasius AL, Giurisato E, Cella M, Schreiber RD, Shaw AS, Colonna M. Bone marrow stromal cell antigen 2 is a specific marker of type I IFN-producing cells in the naive mouse, but a promiscuous cell surface antigen following IFN stimulation. J Immunol. 2006 Sep 1;177(5):3260-5.(440c, FC, PubMed)

Blasius AL, Cella M, Maldonado J, Takai T, Colonna M. Siglec-H is an IPC-specific receptor that modulates type I IFN secretion through DAP12. Blood. 2006 Mar 15;107(6):2474-6. Epub 2005 Nov 17.(440c, FC, PubMed)

Kreisel FH, Blasius A, Kreisel D, Colonna M, Cella M. Interferon-producing cells develop from murine CD31(high)/Ly6C(-) marrow progenitors. Cell Immunol. 2006 Aug;242(2):91-8.

Related Products

Cat. 12-3171    Phycoerythrin (PE) anti-mouse PDCA-1 (plasmacytoid DC Ag-1, pDC Ag-1, BST2, CD317) (clone eBio129c (129c))
Cat. 51-3172    Alexa Fluor® 647 anti-mouse PDCA-1 (plasmacytoid DC (pDC) Ag-1, BST2, CD317, plasmacytoid DC) (clone eBio927)
Cat. 11-4031    Fluorescein isothiocyanate (FITC) Rat IgG2b Isotype Control

Pat. No. pending