Also known as: Platelet-Endothelial Cell Adhesion Molecule 1, PE-CAM1
RUO: For Research Use Only
Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.5 µg of Anti-Mouse CD31 (PECAM-1) FITC (filled histogram). Total viable cells were used for analysis.
Contents: Anti-Mouse CD31 (PECAM-1) FITC Catalog Number: 11-0311 Concentration: 0.5 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: 390 Host/Isotype: Rat IgG2a, κ
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11-0311-81
50 ug
11-0311-82
100 ug
11-0311-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The 390 monoclonal antibody reacts with mouse CD31, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1) and gpIIa. This 130-140 kDa surface protein is expressed by endothelial cells and at low levels on all leukocytes and platelets. It has been reported that CD38 binds to CD31. Homotypic interaction of CD31 is important in adhesion, cell-cell and cell-matrix interaction, and signal transduction.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 390 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 390 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 1.0 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Sadri N, Schneider RJ. Auf1/Hnrnpd-Deficient Mice Develop Pruritic Inflammatory Skin Disease. J Invest Dermatol. 2008 Oct 2. [Epub ahead of print]. (390, IHC frozen, PubMed)
Cao G, O'Brien CD, Zhou Z, Sanders SM, Greenbaum JN, Makrigiannakis A, DeLisser HM. Involvement of human PECAM-1 in angiogenesis and in vitro endothelial cell migration. Am J Physiol Cell Physiol. 2002 May;282(5):C1181-90. (390, IHC frozen)
Wiewrodt R, Anu P. Thomas, Luca Cipelletti, Melpo Christofidou-Solomidou, David A. Weitz, Sheldon I. Feinstein, David Schaffer, Steven M. Albelda, Michael Koval, and Vladimir R. Muzykantov. Size-dependent intracellular immunotargeting of therapeutic cargoes into endothelial cells.Blood. 2002 Feb 1;99(3):912-22.
Horenstein AL, Stockinger H, Imhof BA, Malavasi F. CD38 binding to human myeloid cells is mediated by mouse and human CD31. Biochem J. 1998 Mar 15;330 ( Pt 3):1129-35.
