Contents: Fluorescein isothiocyanate (FITC) anti-human CD29 (Integrin β1)
Catalog Number: 11-0297
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MEM-101A
Isotype: Mouse IgG1
HLDA No.: VI
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Surface staining of normal human peripheral blood cells with anti-human CD29 (MEM-101A) PE (left), and PE-Cy5 (right). Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.
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Description
The MEM-101A monoclonal antibody reacts with human CD29, an approximately 130 kDa integrin β
1. CD29 complexes with one of nine integrin α subunits to form the very late antigen (VLA) subfamily of adhesion molecules. CD29 is expressed broadly on lymphocytes, monocytes and granulocytes.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The MEM-101A antibody has been reported for use in flow cytometric analysis.
Applications Tested
The MEM-101A antibody has been pre-titrated and tested by flow cytometry analysis of human peripheral blood leukocytes. This can be used at 5 μl (1 μg) per 100μl blood (or per 1 million cells in 100 μl total staining volume).
References
Kishimoto, T., A.E.G., von dem Borne, et al. eds. 1998. Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London.
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Human Cellular Antigens Chart