Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD28 (costimulatory)
Catalog Number: 11-0289
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: CD28.2
Isotype: Mouse IgG1, κ
HLDA No.: V 5T CD28.05

CD28 expression on human peripheral blood lymphocytes was determined usingFITC anti-human CD28 (CD28.2). The dotted line shows autofluorescence and the bold histogram shows the staining of peripheral T cells with anti-CD28.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0289	FITC anti-human CD28 (costimulatory)	488	518	FC
12-0289	PE anti-human CD28 (costimulatory)	488	575	FC
13-0289	Biotin anti-human CD28 (costimulatory)	N/A	N/A	FC
14-0289	Affinity Purified anti-human CD28 (costimulatory)	N/A	N/A	FA FC IHC IP
16-0289	Functional Grade* Purified anti-human CD28 (costimulatory)	N/A	N/A	FA FC
17-0289	APC anti-human CD28 (costimulatory)	633	660	FC
25-0289	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD28 (costimulatory)	488	760	FC
45-0289	PerCP-Cy5.5 anti-human CD28 (costimulatory)	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The CD28.2 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The CD28.2 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (1 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Schlossman, S., L. Bloumsell, et al. eds. 1995.Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control