Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-mouse/human/rat CD27
Catalog Number: 11-0271
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: LG.7F9
Isotype: Armenian Hamster IgG
HLDA No.: N/A

Staining of mouse splenocytes with PE anti-human/mouse CD45R(B220)(RA3-6B2)(cat.12-0452) and 0.25 µg FITC hamster IgG isotype control (cat.11-4888) (left) or 0.25 µg FITC anti-human/mouse/rat CD27 (LG.7F9) (right). Total viable cells were analyzed.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0271	Affinity Purified anti-mouse, human, rat CD27	N/A	N/A	FA FC IP
16-0271	Functional Grade* Purified anti-mouse, human, rat CD27	N/A	N/A	FA FC
13-0271	Biotin anti-mouse, human, rat CD27	N/A	N/A	FC
11-0271	FITC anti-mouse, human, rat CD27	488	518	FC
12-0271	PE anti-mouse, human, rat CD27	488	575	FC
17-0271	APC anti-mouse, human, rat CD27	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46^-CD8^- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4⁺ and CD8⁺ effector cells. LG.7F9 cross-reacts with human and rat CD27.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The LG.7F9 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The LG.7F9 antibody has been tested by flow cytometric analysis of mouse splenocyte cell suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Gravestein LA, Nieland JD, Kruisbeek AM, Borst J. 1995. Novel mAbs reveal potent co-stimulatory activity of murine CD27. International Immunology 7(4): 551-557
Tesselaar K, Gravenstine LA, et al. 1997. Characterization of Murine CD70, the Ligand of the TNF Receptor Family Member CD27. The Journal of Immunology 159: 4959-4965
Tesselaar K, Xiao Y, et al. 2002. Expression of the Murine CD27 Ligand CD70 in Vitro and In Vivo. The Journal of Immunology 170: 33-40

Related Products

Cat. 11-4888 Fluorescein isothiocyanate (FITC) Armenian Hamster IgG Isotype Control (clone eBio299Arm)