Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD25 (interleukin-2 receptor, IL-2R)
Catalog Number: 11-0259
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: BC96
Isotype: Mouse IgG1, κ
HLDA No.: V T-072

Surface staining of PHA activated human PBMC with FITC anti-human CD25 (BC96). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0259	Affinity Purified anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	N/A	N/A	FC
13-0259	Biotin anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	N/A	N/A	FC
12-0259	PE anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	575	FC
15-0259	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	670	FC
17-0259	Allophycocyanin (APC) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	633	660	FC
25-0259	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	760	FC
53-0259	Alexa Fluor® 488 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	519	FC
45-0259	PerCP-Cy5.5 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R)	488	690	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor α chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor β chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The BC96 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

When staining for CD25 (with energy emission at 518nm), it is preferable to use Alexa Fluor 488 rather than FITC for optimal detection.

References

Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.

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Cat. 11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control