Contents: Fluorescein isothiocyanate (FITC) anti-human CD25 (interleukin-2 receptor, IL-2R)
Catalog Number: 11-0259
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: BC96
Isotype: Mouse IgG1, κ
HLDA No.: V T-072
| Prices for This Product* |
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Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Surface staining of PHA activated human PBMC with FITC anti-human CD25 (BC96). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-0259 |
**May be discontinued; see superior product Alexa Fluor® 488** FITC anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
518 |
FC
|
| 12-0259 |
PE anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
575 |
FC
|
| 13-0259 |
Biotin anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
N/A |
N/A |
FC
|
| 14-0259 |
Affinity Purified anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
N/A |
N/A |
FC
|
| 15-0259 |
Phycoerythrin-Cy5 (PE-Cy5) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
670 |
FC
|
| 17-0259 |
Allophycocyanin (APC) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
633 |
660 |
FC
|
| 25-0259 |
Phycoerythrin-Cy7 (PE-Cy7) anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
760 |
FC
|
| 45-0259 |
PerCP-Cy5.5 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
690 |
FC
|
| 53-0259 |
Alexa Fluor® 488 anti-human CD25 (Interleukin-2 Receptor, IL-2R, IL2R) |
488 |
519 |
FC
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Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The BC96 monoclonal antibody reacts with human CD25, the 55 kDa interleukin-2 receptor α chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor β chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. CD25 plays a role in lymphocyte differentiating and activation/proliferation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The BC96 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This BC96 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).
When staining for CD25 (with energy emission at 518nm), it is preferable to use Alexa Fluor 488 rather than FITC for optimal detection.
References
Schlossman, S., L. Bloumsell, et al. eds. (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
Related Products
Cat.
11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control
Human Cellular Antigens Chart