Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-mouse CD25 (IL-2 Receptor α, p55)
Catalog Number: 11-0251
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: PC61.5
Isotype: Rat IgG1, λ

Staining of ConA-activated splenocytes with PE anti-CD3e (145-2C11)(cat.12-0031) and 0.125 μg FITC rat IgG1 isotype control (cat.11-4301) (left) or 0.125 µg FITC anti-mouse CD25 (PC61.5) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-0251	PE anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra)	488	575	FC
13-0251	Biotin anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra)	N/A	N/A	FC
14-0251	Affinity Purified anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra)	N/A	N/A	FC IHC IP
15-0251	PE-Cy5 anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra)	488	670	FC
16-0251	Functional Grade* Purified anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra)	N/A	N/A	FA FC
17-0251	APC anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra)	633	660	FC
25-0251	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra)	488	760	FC
27-0251	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55) (APC-Cy7 Equivalent)	633	779	FC
36-0251	Functional Grade* Biotin anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra)	N/A	N/A	FC
45-0251	PerCP-Cy5.5 anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55)	488	690	FC
53-0251	Alexa Fluor® 488 anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra)	488	519	FC
56-0251	Alexa Fluor® 700 anti-mouse CD25 (Interleukin-2 Receptor alpha, IL-2 Receptor alpha, IL-2Ra, p55, IL2Ra) (APC-Cy5.5 Equivalent)	633	723	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor α chain (IL-2Rα). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common γ chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The PC61.5 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The PC61.5 antibody has been tested by flow cytometric analysis of mouse splenocyte cell suspensions and ConA-activated splenocyte culture. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

It is recommended that the Alexa Fluor488 PC61.5 (cat. 53-0251) be used to stain resting cells while the FITC is an excellent choice for activated cells. For more information please contact our Technical Support Department at email tech@ebioscience.com.

References

Lowenthal, J. W., P. Corthesy, et al. (1985)."High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61." J Immunol 135(6): 3988-94.

Lowenthal, J. W., C. Tougne, et al. (1985). "Antigenic stimulation regulates the expression of IL 2 receptors in a cytolytic T lymphocyte clone." J Immunol 134(2): 931-9.

Lowenthal, J. W., R. H. Zubler, et al. (1985)."Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes." Nature 315(6021): 669-72.

Hayashi, T, Hasegawa, K, Adachi, C. (2005) Elimination of CD4+CD25+ T cell accelerates the development of glomerulonephritis during the preactive phase in autoimmune-prone female NZB × NZW F1 mice. Int J Exp Path 86: 289-296 (PC61.5, in vivo depletion, PubMed)

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