Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD24 (Heat Stable Antigen)
Catalog Number: 11-0247
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioSN3 (SN3 A5-2H10)
Isotype: Mouse IgG1
HLDA No.: IV

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0247	Affinity Purified anti-human CD24 (Heat Stable Antigen)	N/A	N/A	FC
13-0247	Biotin anti-human CD24 (Heat Stable Antigen)	N/A	N/A	FC
11-0247	Fluorescein isothiocyanate (FITC) anti-human CD24 (Heat Stable Antigen)	488	518	FC
12-0247	Phycoerythrin (PE) anti-human CD24 (Heat Stable Antigen)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The SN3 monoclonal antibody reacts with the human CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. It has been reported that P-selectin (CD62P) binds to CD24.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The SN3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioSN3 (SN3 A5-2H10) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-0241  FITC anti-mouse CD24 (Heat Stable Antigen) (clone 30-F1)
Cat. 11-0242  FITC anti-mouse CD24 (Heat Stable Antigen) (clone M1/69)
Cat. 11-4714  Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control