FITC anti-mouse CD24 (HAS, Heat Stable Antigen) antibody: Fluorescein conjugate

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Contents: Fluorescein isothiocyanate (FITC) anti-mouse CD24 (Heat Stable Antigen)
Catalog Number: 11-0241
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 30-F1
Isotype: Rat IgG2c, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
11-0241-81	50 ug	$85
11-0241-82	100 ug	$120
11-0241-85	500 ug	$285

*International customers: Please contact your distributor for region specific pricing.

Staining of C57Bl/6 splenocytes with APC anti-mouse CD3e (145-2C11) (cat. 17-0031) and 0.5 μg of FITC Rat IgG2b Isotype Control (cat. 11-4031) (left) or 0.5 μg of FITC anti-mouse CD24 (30-F1) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0241	FITC anti-mouse CD24 (Heat Stable Antigen)	488	518	FC
12-0241	PE anti-mouse CD24 (Heat Stable Antigen)	488	575	FC
14-0241	Affinity Purified anti-mouse CD24 (Heat Stable Antigen)	N/A	N/A	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 30-F1 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. The expression of CD24 detected by 30-F1 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and different antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 30-F1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 30-F1 antibody has been tested by flow cytometric analysis of mouse splenocyte and bone marrow cell suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ledbetter, J. A. and L. A. Herzenberg (1979). "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." Immunol Rev 47: 63-90.
Hardy, R. R., C. E. Carmack, et al. (1991). "Resolution and characterization of pro-B and pre-pro-B cell stages in normal mouse bone marrow." J Exp Med 173(5): 1213-25.

Related Products

Cat. 11-0242    FITC anti-mouse CD24 (Heat Stable Antigen) (clone M1/69)
Cat. 12-0242    PE anti-mouse CD24 (Heat Stable Antigen) (clone M1/69)
Cat. 13-0242    Biotin anti-mouse CD24 (Heat Stable Antigen) (clone M1/69)
Cat. 14-0242    Affinity Purified anti-mouse CD24 (Heat Stable Antigen) (clone M1/69)
Cat. 15-0242    Phycoerythrin-Cy5 (PE-Cy5) anti-mouse CD24 (Heat Stable Antigen) (clone M1/69)
Cat. 16-0242    Functional Grade Purified anti-mouse CD24 (Heat Stable Antigen) (clone M1/69)