Contents: Fluorescein isothiocyanate (FITC) anti-mouse CD22
Catalog Number: 11-0221
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 2D6
Isotype: Rat IgG1, κ
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Staining of BALB/c splenocytes with PE anti-mouse CD19 (MB19-1) (cat. 12-0191) and 0.06 μg of FITC Rat IgG1 Iso Cntrl (cat. 11-4301) (left) or 0.06 μg of FITC anti-mouse CD22 (2D6) (right). Cells in the lymphocyte gate were used for analysis.
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Description
The 2D6 monoclonal antibody reacts with mouse CD22, which is a member of the Siglec (sialic acid-binding immunoglobulin-like lectin) family. CD22 is a 150 kDa type I membrane protein expressed by the B-cell lineage in a developmentally-regulated pattern. In mouse, CD22 is expressed at low levels on the surface of pre-B cells and immature (B220
loIgM
hi) B cells, but high levels on mature (B220
hiIgM
intIgD
+) B cells in the bone marrow. In the periphery, mouse CD22 is highly expressed on all B-cell subsets. Furthermore, mouse CD22 expression is up-regulated slightly upon activation, however expression is lost upon differentiation to a plasma cell. CD22 plays a role in the negative regulation of B-cell Receptor (BCR) signaling, however it has also been demonstrated to function as an intercellular adhesion molecule through binding of sialic acid-bearing proteins expressed by lymphocytes, neutrophils, monocytes and non-hematopoietic cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 2D6 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 2D6 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Symington FW, Subbarao B, Mosier DE, Sprent J.
Lyb-8.2: A new B cell antigen defined and characterized with a monoclonal antibody.
Immunogenetics. 1982;16(5):381-91.
Torres RM, Law CL, Santos-Argumedo L, Kirkham PA, Grabstein K, Parkhouse RM, Clark EA.
Identification and characterization of the murine homologue of CD22, a B lymphocyte-restricted adhesion molecule.
J Immunol. 1992 Oct 15;149(8):2641-9.
Doody GM, Justement LB, Delibrias CC, Matthews RJ, Lin J, Thomas ML, Fearon DT.
A role in B cell activation for CD22 and the protein tyrosine phosphatase SHP.
Science. 1995 Jul 14;269(5221):242-4.
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