FITC anti-mouse CD21/CD35 (CR2/CR1) antibody: Fluorescein conjugate

Contents: Fluorescein isothiocyanate (FITC) anti-mouse CD21/CD35 (CR2/CR1)
Catalog Number: 11-0211
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio8D9 (8D9)
Isotype: Rat IgG2a, λ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
11-0211-81	50 ug	$85
11-0211-82	100 ug	$160
11-0211-85	500 ug	$280

*International customers: Please contact your distributor for region specific pricing.

Staining of BALB/c splenocytes with APC anti-mo/hu B220 (RA3-6B2) (cat. 17-0452) and 0.125 μg of FITC Rat IgG2a Iso Cntrl (cat. 11-4321) (left) or 0.125 μg of FITC anti-mouse CD21/CD35 (eBio8D9) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0211	Affinity Purified anti-mouse CD21/CD35 (CR2/CR1)	N/A	N/A	FA FC WB
16-0211	Functional Grade* Purified anti-mouse CD21/CD35 (CR2/CR1)	N/A	N/A	FA FC
13-0211	Biotin anti-mouse CD21/CD35 (CR2/CR1)	N/A	N/A	FC
11-0211	Fluorescein isothiocyanate (FITC) anti-mouse CD21/CD35 (CR2/CR1)	488	518	FC
12-0211	Phycoerythrin (PE) anti-mouse CD21/CD35 (CR2/CR1)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal antibody eBio8D9 reacts with an eptiope shared by mouse CD21 (CR2) and CD35 (CR1). CD21 and CD35 are alternatively spliced transcripts from the Cr2 gene, which result in cell-surface proteins of 145 and 190 kDa, respectively. CD21 and CD35 are expressed by mature B cells, but not on thymocytes, peripheral T cells, erythrocytes or platelets. Furthermore, there is little evidence which demonstrates their expression on macrophages. CD21 is a receptor for complement component C3d and Epstein-Barr virus (EBV), and in association with CD19 and CD81, CD21 also participates in B-cell activation through the B cell receptor. Cr2 deficient mice display impaired inflammatory and humoral immune responses in vivo. The anti-mouse CD21/35 monoclonal antibody clones eBio4E3 and eBio8D9 do not cross-block each other suggesting that they bind to different epitopes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio8D9 (8D9) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio8D9 (8D9) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kozono Y, Abe R, Kozono H, Kelly RG, Azuma T, and Holers VM. 1998. Cross-linking CD21/CD35 or CD19 increases both B7-1 and B7-2 expression on murine splenic B cells. Journal of Immunology. 160: 1565-1572. (8D9, FA, PubMed)

Martin, B.K., and J.H. Weis. 1993. Murine macrophages lack expression of the Cr2-145 (CR2) and Cr2-190 (CR1) gene products. Eur. J. Immunol. 23: 3037-3042.

Related Products

Cat. 11-0212 Fluorescein isothiocyanate (FITC) anti-mouse CD21/CD35 (CR2/CR1) (clone eBio4E3 (4E3))
Cat. 11-4321 FITC Rat IgG2a Isotype Control