Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD18 (Integrin β₂)
Catalog Number: 11-0187
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: CLB-LFA-1/1
Isotype: Mouse IgG1
HLDA No.: II

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0187	Affinity Purified anti-human CD18 (Integrin beta 2)	N/A	N/A	FC
11-0187	Fluorescein isothiocyanate (FITC) anti-human CD18 (Integrin beta 2)	488	518	FC
12-0187	Phycoerythrin (PE) anti-human CD18 (Integrin beta 2)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The CLB-LFA-1/1 monoclonal antibody reacts with human CD18, also known as β₂ integrin. CD18 is a 90-95 kDa type I transmembrane protein and associates with the α chain of CD11a, CD11b and CD11c to make a heterodimer. The CD18/CD11a complex is also known as lymphocyte function-associated antigen-1 (LFA-1) and plays an important role in cellular adhesion and inflammatory reactions. CD18 is expressed broadly by human lymphocytes and monocytes. Peripheral blood granulocytes express CD18 at lower densities.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The CLB-LFA-1/1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The CLB-LFA-1/1 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 μl (1 μg) per 100μl blood (or per 1 million cells in 100 μl total staining volume).

References

Reinherz, E. L., B. F. Haynes, L. M. Nadler, I. D. Bernstein eds. 1986. Leukocyte Typing II, Springer-Verlag Inc., New York.

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