FITC anti-human CD16 antibody: Fluorescein conjugate

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Contents: Fluorescein isothiocyanate (FITC) anti-human CD16
Catalog Number: 11-0168
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioCB16 (CB16)
Isotype: Mouse IgG1

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
11-0168-71	25 tests	$90
11-0168-73	100 tests	$200

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with 0.125 μg of FITC Mouse IgG1, K Iso Cntrl (cat. 11-4714) (open histogram) or FITC anti-human CD16 (CB16) (colored histogram). Cells in the lymphocyte (left) and granulocyte (right) gates were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0168	Fluorescein isothiocyanate (FITC) anti-human CD16	488	518	FC
12-0168	Phycoerythrin (PE) anti-human CD16	488	575	FC
13-0168	Biotin anti-human CD16	N/A	N/A	FC
14-0168	Affinity Purified anti-human CD16	N/A	N/A	FC
16-0168	Functional Grade* Purified anti-human CD16	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioCB16 monoclonal antibody recognizes CD16 (FcγRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (FcγRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (FcγRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioCB16 (CB16) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioCB16 (CB16) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.125 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Deaglio S, Zubiaur M, Gregorini A, Bottarel F, Ausiello CM, Dianzani U, Sancho J, Malavasi F. Human CD38 and CD16 are functionally dependent and physically associated in natural killer cells. Blood. 2002 Apr 1;99(7):2490-8. (CB16, FC, PubMed)

Zilber MT, Gregory S, Mallone R, Deaglio S, Malavasi F, Charron D, Gelin C. CD38 expressed on human monocytes: a coaccessory molecule in the superantigen-induced proliferation. Proc Natl Acad Sci U S A. 2000 Mar 14;97(6):2840-5. (CB16, Cell Separation, PubMed)

Wirthmueller U, Kurosaki T, Murakami MS, Ravetch JV. Signal transduction by Fc gamma RIII (CD16) is mediated through the gamma chain. J Exp Med. 1992 May 1;175(5):1381-90.

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Cat. 11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control
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