Contents: Fluorescein isothiocyanate (FITC) anti-human CD15
Catalog Number: 11-0159
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN3, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HI98
Isotype: Mouse IgM, κ
HLDA No.: IV M141
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Surface staining of normal human peripheral blood cells with anti-human CD15 (HI98) FITC. Autofluorescence is indicated by open histogram. Cells in the granulocyte population were used for analysis.
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Description
The HI98 monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The HI98 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The HI98 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.125 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).
References
Knapp, W., B. Dorken, et al. eds. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
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