Staining of normal human peripheral blood cells with Mouse IgG1 κ Isotype Control FITC (cat. 11-4714) (open histogram) or Anti-Human CD14 FITC (filled histogram). Cells in the monocyte gate were used for analysis.
Contents: Anti-Human CD14 FITC Catalog Number: 11-0149 Concentration: 5 μl (1 μg)/test Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3, 0.2% BSA Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: 61D3 Host/Isotype: Mouse IgG1, κ HLDA Workshop: V MA085
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11-0149-41
25 tests
11-0149-42
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 61D3 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 61D3 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 μl (1 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York. Kishimoto, T., A.E.G., von dem Borne, et al. eds. (1998). Leucocyte Typing VI: White Cell Differentiation Antigens. Garland Publishing Inc. London. Fadok VA, Warner ML, Bratton DL, Henson PM. CD36 is required for phagocytosis of apoptotic cells by human macrophages that use either a phosphatidylserine receptor or the vitronectin receptor (alpha v beta 3). J Immunol 1998 Dec 1;161(11):6250-7.
