Also known as: 'Integrin alpha X, Integrin aX, ITGAX, p150/95, leu M5 alpha
RUO: For Research Use Only
Staining of normal human peripheral blood cells with Mouse IgG1 κ Isotype Control FITC (cat. 11-4714) (open histogram) or Anti-Human CD11c FITC (filled histogram). Cells in the monocyte gate were used for analysis.
Contents: Anti-Human CD11c FITC Catalog Number: 11-0116 Concentration: 20 μl (1 μg)/test Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: 3.9 Host/Isotype: Mouse IgG1, k HLDA Workshop: III NL707
Product Options
Cat. No.
Size
Price
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11-0116-71
25 tests
11-0116-73
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The 3.9 monoclonal antibody reacts with human CD11c, the 150 kDa integrin αX chain. CD11c non-covalently associates with β2 integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells and subset of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 3.9 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 3.9 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (1μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
McMichael, A.J., P.C.L. Beverly, et al. eds. (1987). Leucocyte Typing III: White Cell Differentiation Antigens. Oxford University Press. New York.
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
