Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD11b (Integrin α_M, Mac-1) - activation epitope
Catalog Number: 11-0113
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: CBRM1/5
Isotype: Mouse IgG1, κ

Staining of 5-minute PMA (50 ng/ml) / ionomycin (1 ug/ml)-stimulated normal human peripheral blood cells. Cells were stained with FITC Mouse IgG1, K Iso Cntrl (cat. 11-4714) (open histogram) or FITC anti-human CD11b (CBRM1/5) (colored histogram). Cells in the granulocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0113	FITC anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope	488	518	FC
12-0113	PE anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope	488	575	FC
14-0113	Affinity Purified anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope	N/A	N/A	FA FC IP
16-0113	Functional Grade* Purified anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope	N/A	N/A	FA FC
51-0113	Alexa Fluor® 647 anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope	633	668	FC
56-0113	Alexa Fluor® 700 anti-human CD11b (Integrin alpha M, Mac-1) - activation epitope (APC-Cy5.5 Equivalent)	633	723	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The CBRM1/5 monoclonal antibody reacts with an activation-specific epitope of human Mac-1. CBRM1/5 binds a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorbol esters but does not recognize Mac-1 on resting myeloid cells. Through interactions with its ligands, Mac-1 participates in adhesive cell interactions. The epitope recognized by this mAb localizes to the I domain on the α chain of Mac-1 very close to the ligand binding site in a region that is widely exposed. CBRM1/5 blocks Mac-1 dependent adhesion to fibrinogen and ICAM-1 and inhibits chemoattractant-stimulated adhesion of eosinophils to the Intercellular Adhesion Molecule-1 (ICAM-1).

Applications Reported

For research use only, not for diagnostic or therapeutic use. The CBRM1/5 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The CBRM1/5 antibody has been pre-titrated and tested by flow cytometric analysis of resting and activated human peripheral leukocytes. This can be used at 20 μl (1 μg) per 100μl blood (or per 1 million cells in 100 μl total staining volume).

References

Oxvig, C., C. Lu, et al. 1999. Conformational changes in tertiary structure near the ligand binding site of an integrin I domain. Proc Natl Acad Sci U S A 96(5): 2215-20.
Weber, C., J. Kitayama, et al. 1996. Differential regulation of beta 1 and beta 2 integrin avidity by chemoattractants in eosinophils. Proc Natl Acad Sci U S A 93(20): 10939-44.
Diamond, M. S. and T. A. Springer 1993. A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen. J Cell Biol 120(2): 545-56.

Related Products

Cat. 11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control