Contents: Fluorescein isothiocyanate (FITC) anti-mouse CD9 Catalog Number: 11-0091 Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Clone: eBioKMC8 (KMC8) Host/Isotype: Rat IgG2a, κ Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
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11-0091-81
50 ug
11-0091-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The eBioKMC8 monoclonal antibody reacts with mouse CD9, a 24 kDa member of the transmembrane 4 superfamily. This family is characterized by the presence of four hydrophobic domains spanning the cell membrane and short N- and C-terminal cytoplasmic domains. CD9 is expressed by several cell types including monocytes, macrophages, platelets, early B cells, activated B and T cells, dendritic cells, eosinophils, basophils, endothelial cells, myoblasts and neuroblasts. On T cells, CD9 functions as a co-stimulatory molecule on naïve T cells. Furthermore, CD9 is expressed in oocytes, and CD9-deficiency results in sterility caused by defective gamete fusion. In mouse macrophages, CD9 functionally associates with FcγRs to modify signals for phagocytosis and inflammatory responses. In mouse B cells, it was discovered that CD9 is a marker for marginal zone B cells, B1 cells, and plasma cells. In dendritic cells, recently it was demonstrated that CD9 facilitates the association of heterologous MHC II molecules. The level of CD9 expression is subject to donor variability.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioKMC8 (KMC8) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBioKMC8 (KMC8) antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Miyake K, Medina KL, Hayashi S, Ono S, Hamaoka T, Kincade PW. Related Articles, Links
Monoclonal antibodies to Pgp-1/CD44 block lympho-hemopoiesis in long-term bone marrow cultures.
J Exp Med. 1990 Feb 1;171(2):477-88. (KMC8, mAb development, PubMed)
Oritani K, Wu X, Medina K, Hudson J, Miyake K, Gimble JM, Burstein SA, Kincade PW.
Antibody ligation of CD9 modifies production of myeloid cells in long-term cultures.
Blood. 1996 Mar 15;87(6):2252-61.
Tachibana I, Hemler ME.
Role of transmembrane 4 superfamily (TM4SF) proteins CD9 and CD81 in muscle cell fusion and myotube maintenance.
J Cell Biol. 1999 Aug 23;146(4):893-904. (KMC8, WB, IP, FA, PubMed)
