Contents: Fluorescein isothiocyanate (FITC) anti-mouse CD8 (beta subunit; CD8β, Ly-3)
Catalog Number: 11-0082
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: CT-CD8b
Isotype: Rat IgG2a
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Staining of C57Bl/6 splenocytes with PE anti-mouse CD3e (145-2C11) (cat. 12-0031) and 0.5 μg of FITC Rat IgG2a Iso Cntrl (cat. 11-4321) (left) or 0.5 μg of FITC anti-mouse CD8b (CT-CD8b) (right). Cells in the lymphocyte gate were used for analysis.
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Description
The CT-CD8b monoclonal antibody reacts with the mouse CD8β molecule. The CD8β chain associates with the CD8α chain to form the CD8α/β heterodimer expressed on the surface of a majority of thymocytes and a subpopulation of mature αβ TCR T cells. However, mature γδ TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 αα homodimers. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The CT-CD8b antibody has been reported for use in flow cytometric analysis.
Applications Tested
The CT-CD8b antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Ledbetter, J. A. and L. A. Herzenberg (1979). "Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens." Immunol Rev 47: 63-90.
Ledbetter, J. A., R. V. Rouse, et al. (1980). "T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views." J Exp Med 152(2): 280-95.
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