Product Information

Contents: Fluorescein isothiocyanate (FITC) anti-human CD5
Catalog Number: 11-0059
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: UCHT2
Isotype: Mouse IgG1, κ
HLDA No.: III 518

Staining of normal human peripheral blood cells with FITC mouse IgG1 isotype control (cat. 11-4719) (open histogram) or FITC UCHT2 (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0059	FITC anti-human CD5	488	518	FC
12-0059	PE anti-human CD5	488	575	FC
25-0059	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD5	488	760	FC
14-0059	Affinity Purified anti-human CD5	N/A	N/A	FC IHC WB
13-0059	Biotin anti-human CD5	N/A	N/A	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The UCHT2 monoclonal antibody reacts with human CD5, a 67 kDa protein expressed by a majority of thymocytes and mature T cells and a subset of B cells. Signaling through the CD5 molecule activates T cells and binding of CD5 to its ligand on B cells, CD72, and plays an important role in T-B interaction and proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The UCHT2 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The UCHT2 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 11-4714 Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control