Fluorescein isothiocyanate (FITC) anti-mouse CD4 (L3T4)

RUO: For Research Use Only

mouse CD4 antibody, Fluorescein isothiocyanate (FITC) conjugate

Staining of mouse thymocytes with PE anti-CD8a (cat.12-0081) (left) or splenocytes with PE anti-CD3e (cat.12-0031) (right) and FITC anti-mouse CD4 (GK1.5). Total viable cells were used for analysis.

Contents: Fluorescein isothiocyanate (FITC) anti-mouse CD4 (L3T4)
Catalog Number: 11-0041
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Clone: GK1.5
Host/Isotype: Rat IgG2b, κ
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
11-0041-81	50 ug	To view relevant pricing, please select your country and resume browsing.
11-0041-82	100 ug
11-0041-85	500 ug
11-0041-86	1 mg

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
11-0042	FITC anti-mouse CD4 (L3T4) (RM4-5)
11-4031	Fluorescein isothiocyanate (FITC) Rat IgG2b Isotype Control
12-0042	PE anti-mouse CD4 (L3T4) (RM4-5)
13-0042	Biotin anti-mouse CD4 (L3T4) (RM4-5)
14-0042	Affinity Purified anti-mouse CD4 (L3T4) (RM4-5)
15-0042	PE-Cy5 anti-mouse CD4 (L3T4) (RM4-5)
16-0042	Functional Grade Purified anti-mouse CD4 (L3T4) (RM4-5)
17-0042	APC anti-mouse CD4 (L3T4) (RM4-5)
25-0042	PE-Cy7 anti-mouse CD4 (L3T4) (RM4-5)
35-0042	PE-Cy5.5 anti-mouse CD4 (L3T4) (RM4-5)

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
11-0041	FITC anti-mouse CD4 (L3T4)	488	518	FC	RUO
12-0041	PE anti-mouse CD4 (L3T4)	488	575	FC	RUO
13-0041	Biotin anti-mouse CD4 (L3T4)	N/A	N/A	FC	RUO
14-0041	Affinity Purified anti-mouse CD4 (L3T4)	N/A	N/A	FC IH/F IP	RUO
15-0041	PE-Cy5 anti-mouse CD4 (L3T4)	488	670	FC	RUO
16-0041	Functional Grade* Purified anti-mouse CD4 (L3T4)	N/A	N/A	FA FC	RUO
17-0041	APC anti-mouse CD4 (L3T4)	633	660	FC	RUO
19-0041	Cy5 anti-mouse CD4 (L3T4)	633	670	FC	RUO
25-0041	PE-Cy7 anti-mouse CD4 (L3T4)	488	760	FC	RUO
36-0041	Functional Grade* Biotin anti-mouse CD4 (L3T4)	N/A	N/A	FC IH/F IP	RUO
51-0041	Alexa Fluor® 647 anti-mouse CD4 (L3T4)	633	668	FC	RUO
53-0041	Alexa Fluor® 488 anti-mouse CD4 (L3T4)	488	519	FC	RUO
56-0041	Alexa Fluor® 700 anti-mouse CD4 (L3T4) (APC-Cy5.5 Equivalent)	633	723	FC	RUO
93-0041	eFluor® 605NC anti-mouse CD4 (L3T4)	350-500	605	FC	RUO
95-0041	eFluor® 650NC anti-mouse CD4 (L3T4)	350-500	650	FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Description

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The GK1.5 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This GK1.5 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, Ishida S. Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. Invest Ophthalmol Vis Sci. 2008 May;49(5):2172-7. (GK1.5, in vivo depletion, PubMed)

Yang Z, Day YJ, Toufektsian MC, Xu Y, Ramos SI, Marshall MA, French BA, Linden J. Myocardial infarct-sparing effect of adenosine A2A receptor activation is due to its action on CD4+ T lymphocytes. Circulation. 2006 Nov 7;114(19):2056-64. (GK1.5, in vivo depletion, PubMed)

Dialynas DP, Quan ZS, Wall KA, Pierres A, Quintáns J, Loken MR, Pierres M, Fitch FW. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983 Nov;131(5):2445-51.

Wilde DB, Marrack P, Kappler J, Dialynas DP, Fitch FW. Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen- specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 1983 Nov;131(5):2178-83.