Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human CD2 FITC (filled histogram). Total viable cells were used for analysis.
Contents: Anti-Human CD2 FITC Catalog Number: 11-0029 Concentration: 20 µl (0.25 µg)/test Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3, 0.2% BSA Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: RPA-2.10 Host/Isotype: Mouse IgG1, κ HLDA Workshop: IV T085
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Size
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11-0029-71
25 tests
11-0029-73
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The RPA-2.10 monoclonal antibody reacts with human CD2, a 50 kDa cell surface receptor expressed by a majority of thymocytes, all mature T cells and subset of NK cells. CD2 is a ligand for CD58 in the human and is involved in adhesion and activation of T cells. RPA-2.10 blocks mixed lymphocyte reaction.
RPA-2.10 crossreacts to primates and pigs.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The RPA-2.10 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This RPA-2.10 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.25 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Kap YS, van Meurs M, van Driel N, Koopman G, Melief MJ, Brok HP, Laman JD, 't Hart BA. A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates. J Histochem Cytochem. 2009 Dec;57(12):1159-67. (RPA-2.10, IHC frozen, primate, PubMed)
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Aversa GG, Bishop GA, Suranyi MG, Hall BM. (1987). RPA-2.10: an anti-CD2 monoclonal antibody that inhibits alloimmune responses and monitors T cell activation. Transplant Proc. 19:277-8
