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Anti-Human CD2 FITC

Also known as: LFA-2, Ly-37

RUO: For Research Use Only
human CD2 antibody, Fluorescein isothiocyanate (FITC) conjugateStaining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human CD2 FITC (filled histogram). Total viable cells were used for analysis.
Contents: Anti-Human CD2 FITC
Catalog Number: 11-0029
Concentration: 20 µl (0.25 µg)/test
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3, 0.2% BSA
Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material.
Clone: RPA-2.10
Host/Isotype: Mouse IgG1, κ
HLDA Workshop: IV T085
 
Product Options
Cat. No. Size Price Add Qty to Cart
11-0029-71 25 tests
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11-0029-73 100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
 
Research Related Products
Cat. No.Format
11-4714 Mouse IgG1 K Isotype Control FITC
 

Available Formats
Cat. No.FormatExcite
(nm)
Emit
(nm)
Reported ApplicationsRegulatory Status
11-0029 Anti-Human CD2 FITC 488 518 FC RUO
12-0029 Anti-Human CD2 PE 488 575 FC RUO
13-0029 Anti-Human CD2 Biotin N/A N/A FC RUO
14-0029 Anti-Human CD2 Purified N/A N/A FA FC IHC (F) WB RUO
15-0029 Anti-Human CD2 PE-Cy5 488 670 FC RUO
16-0029 Anti-Human CD2 Functional Grade* Purified N/A N/A FA FC RUO
17-0029 Anti-Human CD2 APC 633 660 FC RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
 
Description
The RPA-2.10 monoclonal antibody reacts with human CD2, a 50 kDa cell surface receptor expressed by a majority of thymocytes, all mature T cells and subset of NK cells. CD2 is a ligand for CD58 in the human and is involved in adhesion and activation of T cells. RPA-2.10 blocks mixed lymphocyte reaction.

RPA-2.10 crossreacts to primates and pigs.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The RPA-2.10 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This RPA-2.10 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.25 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Kap YS, van Meurs M, van Driel N, Koopman G, Melief MJ, Brok HP, Laman JD, 't Hart BA. A monoclonal antibody selection for immunohistochemical examination of lymphoid tissues from non-human primates. J Histochem Cytochem. 2009 Dec;57(12):1159-67. (RPA-2.10, IHC frozen, primate, PubMed)

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Aversa GG, Bishop GA, Suranyi MG, Hall BM. (1987). RPA-2.10: an anti-CD2 monoclonal antibody that inhibits alloimmune responses and monitors T cell activation. Transplant Proc. 19:277-8

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Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.