Staining of MOLT-4 cells with FITC Mouse IgG1, K Iso Cntrl (cat. 11-4714) (open histogram) or FITC anti-human CD1a (HI149) (colored histogram). Total viable cells were used for analysis.
Contents: Fluorescein isothiocyanate (FITC) anti-human CD1a Catalog Number: 11-0019 Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization Clone: HI149 Host/Isotype: Mouse IgG1, κ HLDA Workshop: V 5T CD01.01 Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
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Size
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11-0019-41
25 tests
11-0019-42
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The HI149 monoclonal antibody reacts with human CD1a, a 49 kDa protein expressed by cortical thymocytes and dendritic cells including Langerhans cells. The CD1 family of proteins share some structural and functional characteristics with the MHC class I molecules; however, members of the CD1 family are not polymorphic. Similar to MHC class I, CD1a associates with the β2-microglobulin and is thought to play a role in antigen presentation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The HI149 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This HI149 antibody has been pre-titrated and tested by flow cytometric analysis of MOLT-4 cells. Please refer to your vial label for the appropriate volume of reagent to use per test (million cells in a 100 μl total staining volume).
References
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York. Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
