Staining of the MOLT-4 cell line with Mouse IgG1 κ Isotype Control FITC (cat. 11-4714) (open histogram) or Anti-Human CD1a FITC (filled histogram). Total viable cells were used for analysis.
Contents: Anti-Human CD1a FITC Catalog Number: 11-0019 Concentration: 5 μl (0.5 μg)/test Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: HI149 Host/Isotype: Mouse IgG1, κ HLDA Workshop: V 5T CD01.01
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Size
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11-0019-41
25 tests
11-0019-42
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The HI149 monoclonal antibody reacts with human CD1a, a 49 kDa protein expressed by cortical thymocytes and dendritic cells including Langerhans cells. The CD1 family of proteins share some structural and functional characteristics with the MHC class I molecules; however, members of the CD1 family are not polymorphic. Similar to MHC class I, CD1a associates with the β2-microglobulin and is thought to play a role in antigen presentation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The HI149 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This HI149 antibody has been pre-titrated and tested by flow cytometric analysis of MOLT-4 cells. This can be used at 5 μl (0.5 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York. Schlossman, S., L. Bloumsell, et al. eds (1995). Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press. New York.
