Staining of normal human peripheral blood cells with 0.25 μg FITC mouse IgG1 (left) or anti-human CD1c (right) and PE anti-human CD19 (HIB19) (cat. 12-0199). Total viable cells were used for this analysis.
Contents: Fluorescein isothiocyanate (FITC) anti-human CD1c (BDCA-1) Catalog Number: 11-0015 Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Clone: L161 Host/Isotype: Mouse IgG1 Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
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Cat. No.
Size
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11-0015-41
25 tests
11-0015-42
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
This L161 monoclonal antibody detects CD1c (also known as BDCA-1), a glycoprotein that is noncovalently linked to beta-2 microglobulin on thymocytes and antigen presenting cells such as dendritic and Langerhans cells. This molecule is also expressed on some circulating and marginal zone B cells, as well as in lymph nodes and germinal centers. CD1c is involved in the presentation of lipid antigens such as microbial fatty acids to effector T cells during the adaptive immune response. Finally, alternative splicing gives rise to three different isoforms of CD1c (soluble, membrane, and cytoplasmic/soluble isoforms).
Applications Reported
For research use only, not for diagnostic or therapeutic use. This L161 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.
Applications Tested
This L161 antibody has been pre-titrated and tested by flow cytometric analysis on normal human peripheral blood cells. This can be used at 5 μl (0.06 μg) per million cells in a 100 μl total staining volume.
References
Barral DC, Brenner MB. CD1 antigen presentation: how it works. Nat Rev Immunol. 2007 Dec;7(12):929-41. Review.
Dzionek A, Fuchs A, Schmidt P, Cremer S, Zysk M, Miltenyi S, Buck DW, Schmitz J.BDCA-2, BDCA-3, and BDCA-4: three markers for distinct subsets of dendritic cells in human peripheral blood. J Immunol. 2000 Dec 1;165(11):6037-46.
del C Salamone M, Mendiguren AK, Salamone GV, Fainboim L. Membrane trafficking of CD1c on activated T cells. J Leukoc Biol. 2001 Oct;70(4):567-77.
Briken V, Jackman RM, Watts GF, Rogers RA, Porcelli SA. Human CD1b and CD1c isoforms survey different intracellular compartments for the presentation of microbial lipid antigens. J Exp Med. 2000 Jul 17;192(2):281-8.
